Introduction to Precision Calibration Standards
Applied Physics Corporation's 4000 Series Dry Monosized Beads represent the pinnacle of precision in calibration standards. These meticulously engineered microspheres deliver exceptional uniformity and accuracy for laboratories and industries requiring NIST-traceable size standards with minimal deviation.
These spherical particles are designed to minimize analytical system response to shape effects and provide researchers and quality control professionals with reliable reference materials for various applications.
Key Features and Benefits of 4000 Series Beads
Superior Spherical Uniformity
Unlike irregularly shaped particles that can produce inconsistent results, the 4000 Series utilizes perfect spheres that ensure consistent measurements across all analytical platforms.
This spherical geometry eliminates shape-related variables that often compromise data accuracy in particle analysis systems.
NIST Traceability for Confidence
Each batch of 4000 Series particles undergoes calibration using Applied Physics' NIST-traceable microscopy methods, providing unmatched confidence in measurement accuracy.
This traceability ensures that all calibrations performed with these standards meet regulatory requirements and scientific best practices.
Precisely Controlled Size Distribution
The 4000 Series beads feature exceptionally narrow standard deviations, with coefficient of variation (CV) values typically between 2.9% and 5.0%.
This tight size distribution is verified through rigorous testing using electrical resistance analysis and optical microscopy, guaranteeing consistency in calibration procedures.
Premium Material Composition
All 4000 Series particles from 200 to 1000μm are manufactured from polystyrene crosslinked with divinylbenzene (PSDVB), creating durable microspheres that maintain their size and shape during handling and use.
This robust composition ensures long-term stability of calibration standards.
Available Size Ranges and Specifications
The 4000 Series offers a comprehensive selection of precisely sized microspheres to meet diverse calibration needs:
| Part Number | Nominal Size | Actual Size | Size Variation (Std Dev, % CV) | Particle Count (per gram) | Package Volume | Price |
|---|---|---|---|---|---|---|
| AP4320A | 200 μm | 200 μm | s = 5.7 μm, 2.9% CV | 2.3 × 10⁵ | 1 Gram | $687.50 |
| AP4324A | 240 μm | 240 μm | s = 7.3 μm, 3.1% CV | 1.3 × 10⁵ | 1 Gram | $687.50 |
| AP4328A | 280 μm | 280 μm | s = 13.5 μm, 4.8% CV | 8.3 × 10⁴ | 1 Gram | $687.50 |
| AP4330A | 300 μm | 300 μm | s = 9.7 μm, 3.4% CV | 4.1 × 10⁴ | 1 Gram | $687.50 |
| AP4340A | 400 μm | 400 μm | s = 12.2 μm, 3.1% CV | 2.8 × 10⁴ | 1 Gram | $687.50 |
| AP4350A | 500 μm | 500 μm | s = 25.1 μm, 5.0% CV | 1.4 × 10⁴ | 1 Gram | $687.50 |
| AP4355A | 550 μm | 550 μm | s = 27.0 μm, 4.9% CV | 1.1 × 10⁴ | 1 Gram | $687.50 |
| AP4365A | 650 μm | 650 μm | s = 24.8 μm, 3.9% CV | 6.6 × 10³ | 1 Gram | $687.50 |
| AP4375A | 750 μm | 750 μm | s = 24.8 μm, 3.9% CV | 3.8 × 10³ | 1 Gram | $687.50 |
| AP4400A | 1000 μm | 1000 μm | s = 48.3 μm, 4.8% CV | 1.6 × 10³ | 1 Gram | $687.50 |
Why Choose Thermo Scientific™ 4000 Series Monosized Beads?
When it comes to precision calibration in particle measurement systems, the Thermo Scientific™ 4000 Series Monosized Beads are considered the best.
These dry beads are highly uniform in size and shape, manufactured with NIST-traceable accuracy, making them the trusted choice for optical particle counters, aerosol instruments, and bio-aerosol sampling devices.
Compared to wet suspensions, these dry beads eliminate the risk of contamination and degradation, resulting in a longer shelf life and consistent performance. They come in a size range from nanometers to microns, with excellent batch-to-batch consistency.
The 4000 Series helps laboratories, researchers, and calibration professionals achieve reliable and reproducible results every time.
Applications Across Industries
Laboratory Calibration and Quality Control
The 4000 Series beads serve as essential calibration tools for particle counters, flow cytometers, and imaging systems in analytical laboratories.
Their precise size distribution makes them ideal for verifying instrument performance and ensuring measurement accuracy.
Pharmaceutical and Medical Device Manufacturing
In pharmaceutical production and medical device manufacturing, these beads provide reliable calibration standards for quality control processes, helping companies
Maintain compliance with regulatory requirements while ensuring product safety and efficacy.
Environmental Monitoring and Research
Environmental scientists rely on the 4000 Series for calibrating particle measurement equipment used in air and water quality assessment.
The precise size standards enable accurate characterization of environmental particulates.
Materials Science and Engineering
Researchers in materials science utilize these microspheres for developing and validating new analytical methods.
As well as for studying particle behavior in various media and conditions.
Quality Assurance and Manufacturing Excellence
Applied Physics Corporation maintains complete control over product quality through its dedicated facilities:
- In-house engineering ensures excellence in design
- Production facilities maintain strict quality standards
- Metrology capabilities verify precise specifications
- The technical support team provides expert assistance
The company’s commitment to quality control and customer service has made the 4000 Series the preferred choice for organizations that require the highest level of precision in their calibration standards.
4000 Series Beads: Chemical & Physical Properties
| Property | Description |
|---|---|
| Material Composition | Polystyrene or Polymer-Based Microspheres (Inert, Non-toxic) |
| Bead Form | Dry (Powder Form), Not in Liquid Suspension |
| Size Range | Approximately from 300 nm (nanometers) to 90 µm (micrometers) |
| Size Uniformity (CV%) | < 5% Coefficient of Variation (High Uniformity) |
| Density | Approximately 1.05 g/cm³ (Depends on polymer type) |
| Refractive Index | Approximately 1.59 at 589 nm (Typical for Polystyrene) |
| Color/Appearance | White to Off-White, Spherical Beads |
| Solubility | Insoluble in Water; Chemically Stable in Most Lab Conditions |
| Thermal Stability | Stable at Room Temperature; Avoid Extreme Heat or Flame |
| Surface Charge | Neutral or Slightly Negative (May vary by batch or customization) |
| Shelf Life | 2+ Years When Stored Properly in Dry, Cool Conditions |
| Traceability | NIST-Traceable for Calibration and Verification Purposes |
4000 Series Beads – Reference Table
| Bead Size | Size in µm (Microns) | Typical Use Case | Comments |
|---|---|---|---|
| Ultra-Fine Beads | 0.3 – 0.9 µm | Aerosol calibration, nanoparticle detection | High precision instruments |
| Fine Beads | 1 – 5 µm | Optical particle counters (OPCs), microscopy | Most commonly used range |
| Medium Beads | 6 – 20 µm | Filter testing, particle sizing systems | Visible under standard microscopes |
| Large Beads | 21 – 50 µm | Air sampler calibration, airflow testing | For larger particle instrumentation |
| Extra-Large Beads | 51 – 90 µm | Spray pattern analysis, airflow visualization | Specialized industrial use |
Ordering Information and Customer Support
Customers can easily order 4000 Series beads in their desired size, with all products packaged as dry spheres in 1-gram quantities. Applied Physics Corporation provides comprehensive technical support for customers seeking guidance on specific applications or special requirements.
For applications beyond the scope of the polymer-based 4000 Series, Applied Physics also offers calibration standards in alternative materials such as silica and glass. Their technical service department can provide detailed information about specialized applications and custom solutions.
Conclusion
The 4000 Series Dry Monosized Beads from Applied Physics Corporation are a reliable and accurate standard for particle size calibration. Their uniform size, NIST traceability, and complete documentation give laboratories and manufacturing facilities confidence in precise measurements and quality control.
By using the 4000 Series, professionals get calibration materials that meet high standards of quality and consistency, backed by Applied Physics Corporation’s strong manufacturing and customer service support.
FAQs
1. Why are these beads called the ‘Gold Standard’ for calibration?
They offer high accuracy, consistency, and reliability in particle size, making them ideal for precise calibration of particle counting instruments.
2. How do these beads improve particle counter performance?
By providing a known reference size, they ensure particle counters measure accurately, enhancing the reliability of contamination monitoring and quality control.
3. What sizes are available in the 4000 Series?
The series typically includes a range of bead sizes, allowing calibration across different particle size detection ranges.
4. Are these beads reusable for multiple calibrations?
Yes, with proper handling and storage, the beads can be reused multiple times without compromising their size uniformity.
5. Where can I purchase genuine 4000 Series Dry Monosized Beads?
They are available through authorized distributors and manufacturers specializing in calibration standards and laboratory supplies.