Applied Physics · Precision technologies since 1992
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Weak Test Lines in Lateral Flow Assays: How Dyed Microspheres Affect Results

Test Lines amid dense red and blue molecule diagrams, showing scattered particles and labeled molecular structures

A weak test line can make a lateral flow assay difficult to interpret, especially when the target is present at a low concentration. The problem may involve the sample, antibodies, membrane, or the colored particles that carry the detection reagent. Dyed polystyrene microspheres can produce visible lines in lateral flow assays, but their color alone […]

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