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5μm to 2000μm: The Ultimate Guide to NIST-Traceable Glass Particle Standards by Applied Physics

Introduction to Glass Particle Standards

Applied Physics Corporation’s 9000 Series Borosilicate Glass Particle Standards represent the gold standard in calibration materials for scientific and industrial applications.

These highly uniform glass microspheres provide researchers and quality control professionals with NIST-traceable standards essential for precise instrument calibration and consistent experimental results.

The comprehensive size range from 5μm to 2000μm makes these standards suitable for diverse applications across multiple industries, from microscopy to advanced materials research. Each particle size is meticulously calibrated using rigorous methodology to ensure exceptional reliability for critical measurement needs.

What Makes the 9000 Series Standards Unique?

NIST Traceability – Unmatched Reliability

The 9000 Series standards are calibrated using Applied Physics’s NIST™-traceable microscopy methods, ensuring an unbroken chain of calibration to national standards.

This traceability is essential for laboratories that need validated measurement protocols and documented quality assurance.

Each package includes a Certificate of Calibration and Traceability to NIST™, which provides:

Superior Physical Properties

Borosilicate Glass (5μm-20μm):

Soda Lime Glass (30μm-2000μm):

Precision Engineering – Consistent Results

The spherical shape of these glass particles is specifically designed to reduce response variation in analytical systems, which can often be affected by irregularly shaped particles.

 This attention to shape ensures that calibration relies on actual size parameters rather than errors caused by different shapes.

What are the specifications of the 9000 series?

SpecificationDetails
MaterialHigh-purity Borosilicate Glass
Particle Size Range2 µm to 200 µm
Size DistributionMonodisperse (narrow size distribution)
ShapeSpherical or near-spherical particles
Refractive Index~1.51 ± 0.01
Density~2.23 g/cm³
Chemical ResistanceHigh resistance to most acids and solvents
Thermal StabilityCan withstand high temperatures without deformation
CertificationsNIST traceable / Certificate of Analysis included
PackagingClean glass vial, sealed to avoid contamination
ApplicationsInstrument calibration, filtration testing, cleanroom validation, metrology
Shelf Life3+ years (under recommended storage conditions).

Complete Size Range and Specifications

Micro Range (5μm-20μm)

The micro range utilizes borosilicate glass for applications requiring a higher refractive index and exceptional chemical stability:

Part#Nominal SizeSize PeakUniformityQuantityPrice
AP90025μm (5 microns)5μm (5 microns)(less than 20%)1 Gram$522.50
APCD90058μm (8 microns)8μm (8 microns)(less than 20%)1 Gram$522.50
AP900815μm (15 microns)15μm (15 microns)(less than 20%)1 Gram$522.50
AP901520μm (20 microns)20μm (20 microns)(less than 20%)1 Gram$522.50

Intermediate Range (30μm-200μm)

The intermediate range uses soda lime glass for excellent optical properties and stability:

Part#Nominal SizeSize PeakUniformityQuantityPrice
AP902030μm (30 microns)30μm (30 microns)(less than 20%)1 Gram$522.50
AP903040μm (40 microns)40μm (40 microns)(less than 20%)1 Gram$522.50
AP904050μm (50 microns)50μm (50 microns)(less than 20%)1 Gram$522.50
AP905060μm (60 microns)60μm (60 microns)(less than 20%)1 Gram$522.50
AP906070μm (70 microns)70μm (70 microns)(less than 20%)1 Gram$522.50
AP907080μm (80 microns)80μm (80 microns)(less than 20%)1 Gram$522.50
AP908090μm (90 microns)90μm (90 microns)(less than 20%)1 Gram$522.50
AP9090100μm (100 microns)100μm (100 microns)(less than 20%)1 Gram$522.50
AP9100110μm (110 microns)110μm (110 microns)(less than 20%)1 Gram$522.50
AP9110120μm (120 microns)120μm (120 microns)(less than 20%)1 Gram$522.50
AP9120130μm (130 microns)130μm (130 microns)(less than 20%)1 Gram$522.50
AP9140140μm (140 microns)140μm (140 microns)(less than 20%)1 Gram$522.50
AP9170170μm (170 microns)170μm (170 microns)(less than 20%)1 Gram$522.50
AP9200200μm (200 microns)200μm (200 microns)(less than 20%)1 Gram$522.50

Macro Range (230μm-2000μm)

The macro range offers larger soda lime glass particles for specialized applications:

Part#Nominal SizeSize PeakUniformityQuantityPrice
AP9230230μm (230 microns)230μm (230 microns)(Less than 20%)1 gram$522.50
AP9280280μm (280 microns)280μm (280 microns)(Less than 20%)1 gram$522.50
AP9330330μm (330 microns)330μm (330 microns)(Less than 20%)1 gram$522.50
AP9400400μm (400 microns)400μm (400 microns)(Less than 20%)1 gram$522.50
AP9480480μm (480 microns)480μm (480 microns)(Less than 20%)1 gram$522.50
AP9550550μm (550 microns)550μm (550 microns)(Less than 20%)1 gram$522.50
AP9650650μm (650 microns)650μm (650 microns)(Less than 20%)1 gram$522.50
AP9750750μm (750 microns)750μm (750 microns)(Less than 20%)1 gram$522.50
AP9950950μm (950 microns)950μm (950 microns)(Less than 20%)1 gram$522.50
AP910001000μm (1000 microns)1000μm (1000 microns)(Less than 20%)1 gram$522.50
AP920002000μm (2000 microns)2000μm (2000 microns)(Less than 20%)1 gram$522.50

Applications in Scientific Fields

Instrument Calibration

These glass particle standards are mostly used for calibrating:

Proper calibration maintains measurement accuracy across all instruments and laboratories, making reproducible research and quality control possible.

Materials Science Research

Researchers in materials science use these standards for:

Colloidal Systems and Suspension Studies

Because of their uniform particle size, these standards are ideal for:

Manufacturing Applications

In industrial settings, these standards are used as:

Benefits of Using 9000 Series Glass

Advantages Over Alternative Materials

Comparison to Polystyrene Standards

While polystyrene particles are commonly used for calibration, glass microspheres offer significant advantages:

FeatureGlass MicrospheresPolystyrene Microspheres
Chemical ResistanceExcellent resistance to solvents and reagentsCan degrade in many organic solvents
Thermal StabilityRemain stable at higher temperaturesLimited temperature range
Mechanical DurabilityHighly resistant to deformationCan be easily deformed
Optical PropertiesStable refractive indexCan absorb certain wavelengths
Long-term StabilityVery minimal degradation over timeMay degrade with extended storage.

Environmental Considerations

Glass microspheres offer some environmental benefits, including:

How are 9000 series glass particle standards calibrated?

The calibration of 9000 Series Glass Particle Standards is a highly precise and controlled process. These particles are of fixed, certified sizes and are used to test the accuracy of scientific instruments such as microscopes, laser particle counters, and calibration systems.

 The particle sample is introduced into the test setup during calibration, and the measured results are compared with the certified size standard. If the values match, the instrument is considered properly calibrated. These particles also come with a NIST-traceable certificate, making every measurement reliable and internationally recognized

Handling and Storage Instructions

Proper Storage Conditions

To maintain the quality of your glass particle standards:

Sample Preparation Guidelines

For best results when using the standards:

Expert Tips for Optimal Use

Maximizing Calibration Accuracy

To achieve the best calibration:

Extending the Utility of Your Standards

Get more value from your glass particle standards:

Conclusion

Applied Physics Corporation’s 9000 Series Borosilicate Glass Particle Standards offer precise, NIST-traceable calibration with exceptional uniformity and reliability. With a wide size range from 5μm to 2000μm, these microspheres ensure accurate, repeatable results for both scientific research and industrial applications — all backed by certified quality and expert support.

For bulk orders, technical inquiries, or to request a full specification sheet, contact our support team today:

FAQs

What are 9000 Series Borosilicate Glass Particle Standards used for?
9000 Series Borosilicate Glass Particle Standards are highly uniform glass microspheres used primarily for the calibration of microscopes, light scattering instruments, and other particle-measuring equipment. They are also utilized in laser light scattering studies, colloidal systems research, and precision spacer applications where uniform bond thickness is needed.

 What sizes and materials are available in the 9000 Series?
The 9000 Series offers particle standards from 5μm to 2000μm. Sizes from 2μm to 20μm are made from borosilicate glass, while sizes from 30μm to 2000μm are made from soda lime glass.

 What is the uniformity and calibration standard for these glass spheres?
Each batch is calibrated using NIST™-traceable microscopy methods to ensure high accuracy. The size uniformity is less than 20% for all products, and optical microscopy is used to measure the size distribution and uniformity.

 Why choose glass microspheres over polystyrene particles?
Glass microspheres provide better chemical and solvent resistance and offer greater mechanical and thermal stability compared to polystyrene spheres. This makes them ideal for demanding sample conditions.

 What documentation is included with each purchase?
Each package comes with a Certificate of Calibration and Traceability to NIST™, a detailed explanation of the calibration method, its uncertainty, a table of chemical and physical properties, and a Material Safety Data Sheet with handling and disposal instructions.

APPLIED PHYSICS TECHNICAL RESOURCE

Continue from the technical question to the application

Wafer inspection, SSIS calibration, particle deposition references, process control and semiconductor measurement workflows. Product and standards references should be interpreted for the actual instrument, facility, protocol and configuration.

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