Step-by-Step Guide to Using the BK-HS32 Nucleic Acid Extraction System

What Is the BK-HS32 Nucleic Acid Extraction System?

The BK-HS32 is an automatic nucleic acid extraction machine that makes the process of isolating DNA or RNA from biological samples easy and fast. It uses magnetic bead-based extraction technology, which extracts 32 samples simultaneously with high purity, repeatability, and efficiency.

Applied Physics automated instrument with touch screen, used for scientific testing and analysis.

Its advanced features make it an excellent choice for clinical labs, research institutions, and pharmaceutical facilities that need high-throughput and accurate nucleic acid extraction.

Key Features and Benefits

  • Processes up to 32 samples at a time
  • Magnetic bead-based method for high accuracy
  • Adjustable temperature range (Room temperature to 100°C)
  • Built-in UV sterilization and HEPA filter for contamination control
  • Easy operation with a 10.1-inch touch screen
  • Pre-installed protocols with storage for over 1000 files
  • Compact design suitable for small lab spaces
  • High magnetic flux of 4500 Gauss for efficient binding and recovery.
BK-HS32 device with 10.1” touchscreen, HEPA filter, UV light, magnetic bead technology, and 32-sample tray

These features are especially useful in:

Step-by-Step Operating Instructions

1. Load Reagents and Samples

  • Open the lid and insert the 96-well deep plate.
  • Add magnetic beads, lysis buffer, wash buffer, and elution buffer to the appropriate wells.
  • Place the biological sample (blood, saliva, swab, etc.) into the designated wells.

2. Attach the Tip Comb

  • Secure the tip comb onto the magnetic rods.
  • Ensure it is properly aligned to avoid disruption during mixing.

3. Select Protocol

  • Turn on the touch screen.
  • From the home screen, choose a pre-saved protocol or load a new one via USB.
  • Review the protocol parameters such as mixing time, temperature, and volume

4. Start the Process

Tap Run

The machine will begin the automated cycle:

  • Lysis: Breaks down cells and releases nucleic acids
  • Binding: Magnetic beads bind with nucleic acids
  • Washing: Removes impurities
  • Elution: Releases purified DNA/RNA into fresh buffer

5. Monitor Progress

  • The screen will show the cycle progress in real time.
  • In case of any error, the system will pause and notify you.

6. End of Extraction

  • Once completed, the machine will alert you.
  • Carefully remove the plate and collect the eluted nucleic acids.

Preparations Before Starting the Machine

Before you begin the extraction process, follow these setup steps:

  1. Power Connection: Plug the BK-HS32 into a stable AC 100–240V power source.
  2. Clean the Workspace: Disinfect your lab bench and wear gloves.
  3. Check Consumables: Have all necessary magnetic beads, reagents, tip combs, and 96-well plates ready.
  4. Inspect the Machine: Make sure the UV lamp is working and the HEPA filter is clean.
  5. Software Setup: Load the appropriate protocol or create a new one using the system touch screen.

Lab tech wearing mask and gloves uses an automated PCR machine to test samples in a lab

Technical Specifications

Specification Details
Sample Capacity 32 samples
Extraction Method Magnetic bead-based
Sample Volume Range 20–1000 μl
Magnetic Flux 4500 Gauss
Extraction Time 15–60 minutes
Temperature Range Room temperature to 100°C
Contamination Control UV lamp (8W), HEPA filter, gate lock
Display Interface 10.1” capacitive touch screen
Protocol Storage Can store over 1000 files
Connectivity 2 USB ports, LAN optional
Dimensions 460 × 470 × 480 mm
Weight 37 kg

post-Extraction Care and Maintenance

Lab worker wearing PPE (mask, gloves, goggles) operating an automated lab cleaning machine; labeled sections: Cleaning, Safety, Discard tip comes here.

  • UV Sterilization: Use the built-in UV light to sterilize the chamber after each run.
  • Clean Rods & Tip Combs: Wipe the magnetic rods and discard used tips.
  • Empty Waste Tray: Regularly dispose of waste liquids and materials.
  • Update Software: Keep your system updated via USB or LAN for better performance.

Safety Tips and Troubleshooting

Safety guidelines sign for BK-HS32 device showing icons and instructions: wear gloves, caution, HEPA filter, UV sterilization, and alert screen, mounted on wall next to the device

  • Always wear gloves and lab coats.
  • Never open the lid during operation.
  • Use only sterile reagents and plates.
  • Replace the HEPA filter as recommended by the manufacturer.

Common Issues and Fixes

Issue Possible Cause Solution
Low yield of nucleic acid Insufficient magnetic bead volume Check reagent levels
Tip comb misalignment Incorrect installation Reattach properly
Machine not starting Loose power cable or protocol error Check connections and restart
Contamination detected UV light or HEPA not working Replace UV lamp or filter

Conclusion

The BK-HS32 Nucleic Acid Extraction System delivers unmatched performance in automated DNA and RNA extraction. Its high-throughput capacity and advanced magnetic bead-based technology ensure reliable, contamination-free, and consistent results across 32 samples simultaneously.

It is easy to use, comes with built-in safety features, and an intelligent software interface, making it ideal for laboratories handling high sample volumes, such as clinical diagnostics, research, and biotechnology. With proper setup, maintenance, and protocol management, this machine can significantly improve your lab’s efficiency and output.

For professionals looking for a compact, high-performing, and future-ready extraction solution, the BK-HS32 is a smart and long-term investment.

FAQs

What type of samples can I process with the BK-HS32?

You can use a variety of biological samples, including blood, saliva, swabs, tissues, and cell cultures for nucleic acid extraction.

Is the BK-HS32 suitable for both DNA and RNA extraction?

Yes. It supports both DNA and RNA extraction depending on the protocol and reagents used.

How many samples can I run in one cycle?

The BK-HS32 supports up to 32 samples in a single run.

How long does one extraction cycle take?

A full extraction cycle can take anywhere from 15 to 60 minutes, depending on sample volume and protocol settings.

Does the machine prevent contamination between samples?

Yes. The system includes HEPA filtration, UV sterilization, and a sealed lid to minimize cross-contamination.

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