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Polystyrene latex beads and particle size standards – Applied Physics

PSL Spheres and polystyrene latex beads from 20nm to 900nm, NIST Traceable, Particle Size Standards

  PSL Spheres are excellent for use with any application that requires a NIST traceable size standard with a very narrow peak standard deviation. These PSL Size Standards are highly uniform polystyrene spheres calibrated within nanometers with NIST traceable methodology. One nanometer is 0.001 µm or 10 Angstroms. SiO2 Process particles in the 5nm and 10nm size range are ideal for the calibration of electron and atomic force microscopes. Polystyrene latex beads are used to produce PSL Wafer Standards to calibrate KLA-Tencor SP1, KLA-Tencor SP2, KLA-Tencor SP3, as well as Hitachi wafer inspection systems, SSIS. PSL Spheres are also used to create aerosol size standards for calibration of size response of laser particle counters and condensation particle counters. Size calibration or wafer inspection systems, also referred to as SSIS or Surface Scanning Inspection Systems is a major requirement in the semiconductor industry. polystyrene latex beads and PSL spheres are also used in laser light scattering studies and colloidal systems research. The 20 nm to 900 nm range of spheres is convenient to support clinical studies for checking the sizes of bacteria, viruses, ribosome and sub-cellular components. PSL Spheres polystyrene latex beads can be ordered by calling Applied Physics at (719) 428-4042. Polystyrene latex beads and PSL spheres are available as uniform polymer spheres in a range of discrete sizes from 20 to 900 nanometers (nm), and also available from 1 micron to 160 microns. The spherical diameters are calibrated with linear dimensions transferred from NIST. Spheres are used instead of irregularly shaped particles to minimize the response of laser scanners which are sensitive to shaped particles. The standards are packaged as aqueous suspensions in 15 milliliter (mL) dropper-tipped bottles. The particle concentrations are optimized for ease of dispersion and colloidal stability. The spheres have a density of 1.05 g/cm3 and an index of refraction of 1.59 @ 589 nm, measured at 25 degrees centigrade. Each bottle of PSL spheres contains a Certificate of Calibration and Traceability to NIST which includes a description of the calibration method and its uncertainty, and a table of chemical and physical properties. A Material Safety Data Sheet, MSDS is available with handling and disposal instructions. polystyrene latex beads are lot-numbered for convenient technical service and support after the sale. PSL Spheres and polystyrene latex beads can be ordered by calling Applied Physics at (719) 428-4042.  

PSL Spheres20nm to 900 nm
Particle CompositionPolystyrene Latex (PSL Spheres)
Particle Density1.05 g/cm³
Index of Refraction1.59 @ 589nm (25°C)
Bottle Size15 mL
Expiration Date≤ 24 months
AdditivesContains trace amounts of surfactant
Suggested Storage Temp.2-8°C
Bottle Size and Volume15ml (A) Bottle
PSL Spheres, 20nm to 900nm, Polystyrene Latex Spheres: 60nm, 100nm and 269nm are useful for NIST Traceable, Calibration Sizes* Sizes similar to NIST SRM Standards
Product Part # Nominal Diameter Certified Mean Peak Size Distribution Solids Concentration
AP3020A     20 nm 20 nm ± 2 nm 1%
AP3030A     30 nm 30 nm ± 1 nm 1%
AP3040A     40 nm 40 nm ± 1 nm 1%
AP3050A     50 nm 46 nm ± 2 nm 1%
AP3060A     60 nm * 60 nm ± 4 nm * 1%
AP3070A     70 nm 70 nm ± 3 nm 1%
AP3080A     80 nm 81 nm ± 3 nm 1%
AP3090A     90 nm 92 nm ± 3 nm 1%
AP3100A     100 nm * 100 nm ± 3 nm * 1%
AP3125A     125 nm 125 nm ± 3 nm 1%
AP3150A     150 nm 147 nm ± 3 nm 1%
AP3200A     200 nm 203 nm ± 5 nm 1%
AP3220A     220 nm 216 nm ± 4 nm 1%
AP3240A     240 nm 240 nm ± 5 nm 1%
AP3269A     269 nm * 269 nm ± 5 nm * 1%
AP3300A     300 nm 296 nm ± 6 nm 1%
AP3350A     350 nm 350 nm ± 6 nm 1%
AP3400A     400 nm 400nm ± 9 nm 1%
AP3450A     450 nm 453 nm ± 9 nm 1%
AP3495A     500 nm 496 nm ± 8 nm 1%
AP3500A     500 nm 498 nm ± 9 nm 1%
APCD3560A     565 nm 565 nm ± 9 nm 1%
AP3600A     600 nm 600 nm ± 9 nm 1%
AP3700A     700 nm 707 nm ± 9 nm 1%
AP3800A     800 nm 799 nm ± 9 nm 1%
AP3900A     900 nm 903 nm ± 12 nm 1%
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