Description
NHS-activated 40 nm gold nanoparticles are provided dried with conjugation kit components that make initial conjugation sweeps easy – no EDC/NHS activation required, just add purified antibody and follow our simple protocol (below).
Kit includes:
- Lyophilized gold nanospheres that reconstitute to 20 OD; 50 µL per small unit and 500 µL per large unit
- Easy-to-use detailed protocol (also available below to view before you buy)
- Desalting buffer for antibody, reaction buffer, quencher, and conjugate diluent
- Amicon® Ultra 0.5 mL spin filters for antibody buffer exchange/desalt
Small reaction kits generally require approximately 2 µg of purified antibody per unit, while the large reaction kit generally requires approximately 25 µg of purified antibody per unit.
Materials are shipped ambient. Store with desiccant at –20°C upon receipt. Avoid freeze-thaw cycles. For Research Use Only.
| Overview | |
|---|---|
| Surface chemistry | N-Hydroxysuccinimide (NHS) |
| Particle form | Powder |
| Specifications | |
| TEM Diameter | 40 ± 4 nm |
| CV | ≤ 15% |
| Documents | |
| Example Certificate of Analysis (CoA) | Download |
| Safety Data Sheet (SDS) | 20 OD (BioReady) |
| Storage & Handling | Download |
| Protocols | Antibody Purification BioReady 40 nm Gold NHS Kit Conjugation |
| Expected Ranges | |
| Peak wavelength (λmax) upon resuspension | 520–550 nm |
Reviews
There are no reviews yet.