The BioReady Reaction Buffers are specialized, pre-formulated buffers designed specifically for the covalent conjugation of antibodies and proteins to nanoparticles (such as nanoComposix’s BioReady Carboxyl Gold Nanoshells). These buffers are engineered to provide the optimal reaction matrix—specifically the correct pH and salt concentrations—required for highly efficient EDC/Sulfo-NHS coupling in lateral flow assay development and other diagnostic applications.
The same high quality conjugation reaction buffers, block buffer, and conjugate diluent for covalent conjugation found in our High Sensitivity Gold Conjugation Kit, now available in catalog and bulk volumes.
BioReady Potassium Phosphate Reaction Buffer
5mM Potassium Phosphate, 0.5% PEG20K, pH 7.4
BioReady Sodium Phosphate Reaction Buffer
5mM Sodium Phosphate, 0.5% PEG20K, pH 7.4
BioReady PBS Reaction Buffer
0.01X PBS, 0.5% PEG20K, pH 7.4
BioReady Desalting Buffer
10mM Potassium Phosphate, pH 7.4
BioReady Block Buffer
4mM Sodium Tetraborate, 1% BSA, 0.05% sodium azide, pH 8.2
BioReady Running Buffer
1X PBS, 1% Tween 20, pH 7.4
BioReady Conjugate Diluent
0.5X PBS, 0.5% BSA, 0.5% casein, 1% tween 20, 0.05% azide, pH 8.0
| Buffer Type | KPhos, NaPhos, PBS |
|---|---|
| Volume | 100 mL, 500 mL, 1 L |
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