Description
The use of microparticles as a solid phase support in various immunoassays and affinity purifications has been established for some time; however, achieving high activity and stable binding of solid phase ligands has often been a major difficulty. Biotinylated compounds become stably bound to Power Bind Streptavidin Coated particles after simple incubation in buffer due to the high affinity of the biotin and streptavidin interaction. The particles also function as a spacer, which improves the specific activity of the bound ligand. These are monodisperse nonmagnetic particle suspensions with streptavidin covalently bound to the surface in a highly active form.
Features:
Made from 0.3μm or 0.85μm Applied Physics USA OptiLink Carboxylate Modified particles
High biotin binding capacity
Extremely stable with a long shelf life
Simplifies the process of binding ligands to particles
Combines the advantages of high surface area with easy, high affinity, and high specific activity binding
Excellent for chemiluminescent assays, biotinylated ligand capture, molecular biology, immunoassays, or sample prep applications
Benefits:
Dissociation constant (Kd 10^-15 molar) with easy one step binding protocols for biotinylated ligands
High activity of surface bound ligands to easily solve difficult coupling problems
Simple aqueous biotinylation reactions
Low nonspecific interactions utilizing a blocked microparticle surface
Highly mobile particles for membrane based applications
Can be used in EIA formats with biotin and enzyme detection systems
Beneficial spacer effect of the SA molecule
A choice of 0.3μm or 0.85μm diameters for different application requirements
Recommended For:
Universal nonmagnetic beads for coupling unique ligands
Clinical diagnostics and molecular biology applications
Product relationship
Available Through Applied Physics
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Catalog relationship: Available Through Applied Physics
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