Description
The use of microparticles as a solid phase support in various immunoassays and affinity purifications has been established for some time; however, achieving high activity and stable binding of solid phase ligands has often been a major difficulty. Biotinylated compounds become stably bound to Power Bind Streptavidin Coated particles after simple incubation in buffer due to the high affinity of the biotin and streptavidin interaction. The particles also function as a spacer, which improves the specific activity of the bound ligand. These are monodisperse nonmagnetic particle suspensions with streptavidin covalently bound to the surface in a highly active form.
Features:
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Made from 0.3μm or 0.85μm Applied Physics USA OptiLink Carboxylate Modified particles
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High biotin binding capacity
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Extremely stable with a long shelf life
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Simplifies the process of binding ligands to particles
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Combines the advantages of high surface area with easy, high affinity, and high specific activity binding
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Excellent for chemiluminescent assays, biotinylated ligand capture, molecular biology, immunoassays, or sample prep applications
Benefits:
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Dissociation constant (Kd 10^-15 molar) with easy one step binding protocols for biotinylated ligands
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High activity of surface bound ligands to easily solve difficult coupling problems
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Simple aqueous biotinylation reactions
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Low nonspecific interactions utilizing a blocked microparticle surface
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Highly mobile particles for membrane based applications
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Can be used in EIA formats with biotin and enzyme detection systems
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Beneficial spacer effect of the SA molecule
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A choice of 0.3μm or 0.85μm diameters for different application requirements
Recommended For:
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Universal nonmagnetic beads for coupling unique ligands
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Clinical diagnostics and molecular biology applications
